Chlamydia trachomatis (CT) DNA

Test code

ICT

Specimen

1 dry genital swab or 20 mL 1st voided urine

Container

ThinPrep or SurePath bottle or sterile bottle for urine

Special instructions

Nil

Collection

Samples in cytology preservative should be collected using a cervical broom and placed in liquid-based cytology fixative

Causes for rejection

Receipt of urine in sterile container >24 hours from collection; transport device with multiple swabs; female urethral swab; bloody or grossly mucoid specimens; bacterial swabs

Description of analysis

Detection of sexually transmitted disease caused by infection of Chlamydia trachomatis (CT) using real time polymerase chain reaction (RT-PCR)

Test methodology

DNA is extracted and purified from sample and is subjected to RT-PCR amplification and detection

Limitation

Above methodology detects the presence of CT DNA but cannot determine the viability status of the bacteria

Storage instruction

Specimen should be kept at room temperature

Reference

Kohl KS. et. al. (2003) Obstet Gynecol Clin North Am. Dec;30 (4): 637-58. Developments in the screening for Chlamydia trachomatis: a review

QUESTIONS?

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