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GenoQuest EGFR real time PCR kit

Kit enables oncology practitioners and researchers to identify 29 mutations in the EGFR pathway implicated in various types of cancer and treatments. 

What is it?

Highly sensitive real time PCR kit
 
This highly sensitive assay enables oncology researchers and practitioners to identify 29 mutations in exons 18, 19, 20 and 21 and develop beneficial therapy for cancer patients. 
 
The epidermal growth factor receptor (EGFR) pathway is implicated in various types of cancer.
 
Patients with EGFR mutations are resistant to EGFR tyrosine kinase inhibitor therapy. 
 
The presence of EGFR mutations have diagnostic significance because mutation tests helps identify more effective therapy for some patients.
 
DiagCor’s fast and cost-effective EGFR real time PCR kit provides a highly sensitive molecular technique to detect as low as 1% of mutant molecules present in high background of normal DNA.

Who is it for?

This kit is especially valuable to oncology practitioners and researchers concerned in identifying and treating various cancers.
 
The GenoQuest EGFR real time PCR kit is also useful to oncology researchers.

Why is it different?

This highly sensitive kit allows selective site-specific detection of mutant DNA.
 
It can detect less than 1% mutant sequence copies against a background of 99% wild-type DNA.
 
The ready-to-use reagent is compatible with various real time PCR machines and delivers results 2 hours after extraction.

Advantages

Accurate – Highly sensitive test allows site-specific detection of less than 1% mutant sequence copies in a background of 99% wild-type DNA
Practical – Detects the full range of mutations located on the EGFR gene
Fast – Delivers results approximately 2 hours after extraction, tests up to 8 samples simultaneously
Easy – No post-PCR manipulation required
Cost effective – Lowest overall cost in market

Need to know

This is a consumable assay kit, developed, supplied and supported by DiagCor. It requires a compatible instrument and a meticulous MDx workflow.

  • Works with

    Applied Biosystems ViiA7

    Applied Biosystems 7500 Fast

    Applied Biosystems 7900

  • Workflow
    Workflow (GenoScreen & GenoQuant)

What you get

In the box

  • Seven duplex (FAM + Cy5) EGFR mutation assays with internal control for each assay
  • Real time master mix
  • DNase free water    

Service and support
The DiagCor support team includes skilled technicians,  engineers and scientists
 
  • Distributor and end-users receive direct training supported by video conferencing
  • Standard operation procedures explain test execution step-by-step 
  • Experienced technicians will familiarize users with procedures by guiding them through a series of trial tests 
 
DiagCor provides extensive training to end users and distributors that covers
 
  • Correct application of the system, including how to deploy in public health programs for best ROI
  • Practical lab technician training 

Equipment required
  • Real-Time PCR machine
  • High speed centrifuge
  • Vortex
  • Pipettes
  • Water bath
  • Ice bucket 

Extraction kits

QIAamp FFPE extraction kit


Analytes
  • EGFR mutation (29 mutations)
  • Internal control
  • NTC control

Specs
Catalogue number R14001
Configuration 24 tests (real time PCR kit)
Detected mutations EGFR mutation (29 mutations) in exon 18, 19, 20 and 21: S7681, T790M, L858R, L861Q, G719A, G719C, G719S, 19 deletions, 3 insertions
Specimens Human genomic DNA extracted from fresh, frozen or paraffin embedded tissue
Internal controls Internal control; NTC control
Reported results Wild-type or mutated EGFR